Escherichia coli and Salmonella typhimurium

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Genome and genetics. The genome. Linkage map of Escherichia coli k-12 edition 7. Linkage of Salmonella typhimurium. Gene-protein index of Escherichia coli K-12, edition 2. Genome organization. Locations of native insertion sequence elements. Selectable phenotypes. Alterations in the genome. Analysis of mutagenesis. General recombination in Escherichia coli. DNA repair. Transposition and site-specific recombination. Uses of transposable elements and maps of known insertions. Gene transfer: conjugation. Structure and function of the factor and mechanism of conjugation. Hfr strains of Escherichia coli k-12. F-mediated conjugation, F+ strains, and Hfr strains of Salmonella typhimurium and Salmonella abony. F-prime and R-prime factors. Gene transfer: transduction. Generalized transduction. Specialized transduction. Gene transfer: transformation. Mechanisms of DNA transformation. Genetic measures of chromosome size. Mapping techniques and determination of chromosome size. Strains and useful strain constructions. Derivations and genotypes of some mutant derivatives of escherichia coli K-12. Salmonella typhimurium strains used in genect analysis. Useful host strains and techniques for recombinant DNA experiments. Regulation of gene expression. General mechanisms. Strategies in regulation of transcription initiation. Transript elongation and termination in Escherichia coli. Transcription attenuation. Translational initiation. Regulation by proteolysis. Regulation of multigene systems. Multigene systems and regulons. Regulation of carbon and nitrogen utilization. Phosphate regulation of gene expression in Escherichia coli. Heat shock response. The S.O.S. response of Escherichia coli. Ribossomes and tRNA. Regulation of the expression of aminoacyl-tRNA synthetases and translation factors. The stringent response. Paradigms of operon regulation. The operon: an historical account. The lactose operon. The Trypthopan operon. The L-arabinose operon. The maltose regulon. The galactose operon. The proline utilization operon. The D-serine deaminase operon. Growth of cells and cultures. Modulation of chemical composition and other parameters of the cell by growth rate. Effect of temperature, pH, water activity, and pressure on growth. Regulation of chromosome replication. Cell division: parameter values and the process. Timing of synthetic activities in the cell cycle. The variability and individuality of the bacterium. Ecology, evolution, and population structure. Colicins and col plasmids. Genectic structure and variation in natural populations of Escherichia coli. Evolutionary history of enteric bacteria