Gene Expression in Neural Tissues

The study of gene expression in neural tissue brings to bear some of the newest and most productive scientific technology to one of the most exciting and fastest growing areas. This volume describes methods for the study of the expression of specific genes frequently found in very low abundance.Key Features* Convenient benchtop format* Methods presented for easy adaptation to new systems* Comprehensive protocols included for* In situ hybridization* Comparison of in situ and immunochemical methods for the study of regulatory molecules* Gene transfer* The study of the regulation of gene expression* The enhancer trap method* Quantifying rare mRNAs* Isolation and use of cell lines* The homology approach for receptor cloning* Trouble Shooting