Nucleophilic active site mimetics and methodological advances in activity-based protein profiling

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The present thesis covers methodological advances in various fields of activity-based protein profiling. An azide-containing trifunctional linker exhibiting a vast background reduction as well as a novel pH-sensitive cleavable protein linker could be established. Furthermore, the ubiquitous CuAAC click chemistry reaction was enhanced through the development of fine tuned copper ligands that extended the scope of application. Finally, a pioneering approach for the activity-based capture of electrophilic natural products via nucleophilic active site mimetics was investigated successfully.