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Improving tobacco (Nicotiana tabacum) suspension cells as a molecular farming platform

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The first endogenous matrix-metalloproteinase NtMMP1 from tobacco BY-2 cells was characterized in detail. Transgenic BY-2 cell lines expressing antisense-RNA against different endogenous proteases (aspartic, cysteine, matrix-metallo and serine) separately (single-antisense) or simultaneously (multi-antisense) was generated. The inhibition of endogenous protease(s) in those lines was verified and the stability of recombinant protein(s) was tested.

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Improving tobacco (Nicotiana tabacum) suspension cells as a molecular farming platform, Manoj Kumar Mandal

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Jaar van publicatie
2010
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(Paperback)
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