Culturing Nerve Cells, Second Edition

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Culture systems for neurons and glia closely resemble their in situ counterparts, allowing for the exploration of significant cell interactions and their underlying mechanisms. This resource serves as a comprehensive manual for culturing nerve cells, offering detailed recipes and protocols while also explaining the principles that guide these methods. Contributors share insights from their laboratories, providing a wealth of information typically gained through direct visits. The introductory chapters outline the fundamental principles necessary for growing nerve cells, while subsequent chapters focus on specific cell types. Authors detail how their experimental objectives influence their culture techniques, discussing the advantages and drawbacks of the systems they have created. They present thorough protocols and describe their cultures practically, from the initial plating of cells to the various stages of development. With contributions from numerous experts in the field, this work not only serves as a practical guide but also enriches the reader's understanding of the science behind nerve cell culture, making it an invaluable resource for researchers and practitioners alike.